![]() Phosphoribosylation of ubiquitin promotes serine ubiquitination and impairs conventional ubiquitination. īhogaraju, S., Kalayil, S., Liu, Y., Bonn, F., Colby, T., Matic, I., & Dikic, I. Inhibition of bacterial ubiquitin ligases by SidJ-calmodulin catalysed glutamylation. Molecular Microbiology, 7, 7-19.īhogaraju, S., Bonn, F., Mukherjee, R., Adams, M., Pfleiderer, M. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. pneumophila, suggesting that host-derived ubiquitin-conjugating enzymes and ubiquitin ligases participate in the translocation of Legionella effector proteins and influence intracellular persistence and survival.īerger, K. In summary, we identified host ubiquitin pathways as important for the efficiency of Dot/Icm effector translocation by L. We further showed that two host factors, the E2 ubiquitin-conjugating enzyme, UBE2E1, and the E3 ubiquitin ligase, CUL7, were important for supporting Dot/Icm translocation and L. Gene set enrichment analysis of siRNAs leading to decreased RalF translocation, showed that ubiquitination was the most highly overrepresented category in the pathway analysis. Following secondary screening, 70 genes were successfully validated as 'high confidence' targets. For the primary screen, we found that silencing of 119 genes led to increased translocation of RalF, while silencing of 321 genes resulted in decreased translocation. Here we employed a high-throughput, genome-wide siRNA screen to systematically test the effect of silencing 18,120 human genes on translocation of the Dot/Icm effector, RalF, into HeLa cells. Since the secretion of effectors via the Dot/Icm system does not occur in the absence of host cells, we hypothesised that host factors actively participate in Dot/Icm effector translocation. Most searched terms about Eliza Ong on Google and Bing are Eliza Ong marriage, Eliza Ong age, Eliza Ong wiki, Eliza Ong photos, Eliza Ong lover, Eliza Ong instagram, Eliza Ong facebook, Eliza Ong family, Eliza Ong salary, Eliza Ong height, Eliza Ong bio, Eliza Ong income, Eliza Ong house, Eliza Ong latest news, Eliza Ong tiktok, Eliza Ong musicaly.The Dot/Icm system of Legionella pneumophila is essential for virulence and delivers a large repertoire of effectors into infected host cells to create the Legionella containing vacuole.What is the age of Eliza Ong?As of 2023, Eliza Ong age is 13 years.When is the birthday of Eliza Ong?, 2010.Where is Eliza Ong from? Melbourne, Australia.Who is the mother of Eliza Ong?Eliza Ong‘ mother name is NA.Who is the father of Eliza Ong?Eliza Ong‘ father name is NA. ![]() ![]()
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